Influence of Low-Frequency Vibrations on Methylene Blue Reduction Process

Authors: Morozov A.N., Fadeev G.N., Bogatov N.A., Boldyrev V.S., Zadorozhny N.A., Timchenko S.L. Published: 23.02.2022
Published in issue: #1(100)/2022  
DOI: 10.18698/1812-3368-2022-1-141-156

Category: Chemistry | Chapter: Physical Chemistry  
Keywords: methylene blue, low-frequency harmonic vibrations, sonochemical process, absorption spectrometry, EPR spectrometry


Methylene blue is known for a wide spectrum of biochemical activity: being reversibly oxidized and reduced, it acts as a hydrogen carrier and can be considered as a model of the active group of the enzyme dehydrase. When harmonic vibrations with a frequency of 7--30 Hz are introduced into an aqueous solution containing methylene blue and ascorbic acid, the kinetics of redox transformations changes: the kinetic dependences of the influence of the acoustic vibrations differ from the data obtained in the absence of low-frequency influences. Under certain conditions, effects such as acceleration and inhibition of the sonochemical process are observed. The paper studies the reaction of hydrogen transfer in the field of low-frequency oscillations. The purpose of the study was to elucidate the role of factors accompanying the effect: we determined the influence of oxygen, the emerging radical forms of methylene blue, and ascorbic acid. By absorption spectroscopy and EPR spectrometry, we specified the parameters of the compounds formed at the intermediate stages of the methylene blue reduction process. Relying on experimental data, we introduce a method for countering the COVID-19 virus using methylene blue

The study was funded by RFBR (project no. 20-33-90152)

Please cite this article in English as:

Morozov A.N., Fadeev G.N., Bogatov N.A., et al. Influence of low-frequency vibrations on methylene blue reduction process. Herald of the Bauman Moscow State Technical University, Series Natural Sciences, 2022, no. 1 (100), pp. 141--156 (in Russ.). DOI: https://doi.org/10.18698/1812-3368-2022-1-141-156


[1] Kutsenko S.A. Fundamentals of toxicology. Biomeditsinskiy zhurnal, 2003, vol. 4, art. 119, pp. 188--284 (in Russ.).

[2] Zubkov A.V., Krayushkin A.I., Zagrebin V.L. Use of methylene blue in intraoperative search of parathyroid glands. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal [International Research Journal], 2018, no. 9-1, pp. 118--128 (in Russ.). DOI: https://doi.org/10.23670/IRJ.2018.75.9.022

[3] Alamdari D.H., Moghaddam A.B., Amini S., et al. Application of methylene blue --- vitamin C --- N-acetylcysteine for treatment to critically COVID-19 patients, report of a phase-1 clinical trial. Eur. J. Pharmacol., 2020, vol. 885, art. 173494. DOI: https://doi.org/10.1016/j.ejphar.2020.173494

[4] Sin’ka lechit [Blue cures]. nplus1.ru: website (in Russ.). Available at: https://nplus1.ru/material/2020/09/01/methylene-blue (accessed: 15.10.2021).

[5] Boldyrev V.S. Deystvie nizkochastotnykh kolebaniy na biokhimicheski aktivnye struktury. Dis. kand. tekh. nauk [Effect of low-frequency oscillations on bioactive structures. Cand. Sci. Eng. Diss.]. Moscow, MUCTR Publ., 2013 (in Russ.).

[6] Fadeev G.N., Boldyrev V.S., Bogatov N.A. Features of energy of chemical reactions under the action of non-lethal acoustic weapons. J. Clin. Sci. Trans. Med., 2020, vol. 2, no. 1, art. 000106.

[7] ‎Pullman B., Pullman A. Quantum biochemistry. Interscience, 1963.

[8] Fadeev G.N., Boldyrev V.S., Averina Yu.M., et al. Adhesive conversion lacquer coatings on magnesium alloys. Tsvetnye metally, 2019, no. 10, pp. 73--77 (in Russ.). DOI: https://doi.org/10.17580/tsm.2019.10.11

[9] Fadeev G.N., Boldyrev V.S., Bogatov N.A., et al. Specifics of reduction-oxidation processes exposed to a low-frequency acoustic field. Herald of the Bauman Moscow State Technical University, Series Natural Sciences, 2020, no. 1 (88), pp. 80--92 (in Russ.). DOI: https://doi.org/10.18698/1812-3368-2020-1-80-92

[10] Terenin A.N. Fotonika molekul krasiteley i rodstvennykh soedineniy [Photonics of dye molecules and related organic compounds]. Leningrad, Nauka Publ., 1967.

[11] Shatskikh T.S. Fotofizicheskie protsessy v gibridnykh assotsiatakh kolloidnykh kvantovykh tochek CdS s molekulami metilenovogo golubogo. Dis. kand. fiz.-mat. nauk [Photophysical processes in hybrid associates of CdS colloidal quantum dots with methylene blue molecules. Cand. Sci. Phys.-Math. Diss.]. Voronezh, VGU Publ., 2014 (in Russ.).

[12] Benson S.W. The foundations of chemical kinetics. McGraw Hill, 1960.

[13] Emanuel N.M., Knorre D.G. Chemical kinetics homogeneous reactions. Wiley, 1973.

[14] Dement’yeva O.Yu., Timchenko S.L., Zadorozhny N.A. Methods for measuring the line width of resonant absorption in EPR. Fizicheskoe obrazovanie v vuzakh [Physics in Higher Education], 2016, vol. 22, no. 4, pp. 77--87 (in Russ.).

[15] Zadorozhny N.A., Timchenko S.L., Yurasov N.I., et al. Electronic paramagnetic resonance in laboratory works for the students studying the physics and chemistry. Sovremennyy fizicheskiy praktikum, 2016, no. 14, pp. 77--79 (in Russ.).

[16] Fernandez-Perez A., Valdes-Solis T., Marban G. Visible light spectroscopic analysis of Methylene Blue in water; the resonance virtual equilibrium hypothesis. Dyes Pigm., 2018, vol. 161, pp. 448--456. DOI: https://doi.org/10.1016/j.dyepig.2018.09.083

[17] Henry M., Summa M., Patrick L., et al. A cohort of cancer patients with no reported cases of SARS-CoV-2 infection: the possible preventive role of Methylene Blue. Substantia, 2020, vol. 4, no. 1, suppl. 1, art. 888. DOI: https://doi.org/10.13128/Substantia-888

[18] Davies M.B., Partridge D.A., Austin J.A. Vitamin C: its chemistry and biochemistry. Royal Society of Chemistry, 1991. DOI: https://doi.org/10.1039/9781847552303